RiboMAX Large Scale RNA Production System-SP6 from Promega

Product Specs
ItemRiboMAX Large Scale RNA Production System-SP6
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1280
Quantity1 system

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Neutralizing antibodies from prior exposure to dengue virus negatively correlate with viremia on re-infectionCommunications MedicineOctober 19, 2023Anbalagan Anantharaj, Tanvi Agrawal, Pooja Kumari Shashi, Alok Tripathi, Parveen Kumar, Imran Khan, Madhu Pareek, Balwant Singh, Chitra Pattabiraman, Saurabh Kumar, et al.linearized using Sac II enzyme and in vitro transcribed using the SP6 RNA polymerase (Promega, Cat no: P1280). The transcript was purified and used as a template for generating standard curve to estimate the copy

(2) NS1 Protein N-Linked Glycosylation Site Affects the Virulence and Pathogenesis of Dengue VirusVaccinesMay 8, 2023Fang E, Li M, Liu X, Hu K, Liu L, Zhang Z, Li X, Peng Q, Li Y.used as a template for in vitro transcription with a RiboMAX Large Scale RNA Production System (Cat. P1280; Promega, Madison, WI, USA). The 5′ end of viral RNA was capped using a Ribo m7G Cap Analog (Cat. P1712;

(3) Construction of a Dengue NanoLuc Reporter Virus for In Vivo Live Imaging in MiceVirusesJune 9, 2022Enyue Fang et al.in vitro ligation was used as a template for in vitro transcription using SP6 Transcription Reagent (Cat. P1280; Promega) and m7G Cap Analog (Cat. P1712; Promega). RNA was purified using the RNeasy MinElute Cleanup

(4) RNA Interference of mRNA Processing Factors in Drosophila S2 CellsmRNA Processing and MetabolismMarch 30, 2022Alicia M. Celotto, Brenton R. GraveleyK4600-01). 6. Transcription reagents: Ribomax Large Scale RNA Production Kits (Promega, SP6 kit, cat. no. P1280, T7 kit, cat. no. P1300). 7. RNA isolation reagents: Trizol™ reagent (Invitrogen). 8. Reverse transcription

(5) Evolutionarily conserved inhibitory uORFs sensitize Hox mRNA translation to start codon selection stringencyProceedings of the National Academy of SciencesMarch 1, 2022Ivaylo P. Ivanov, James A. Saba, Chen-Ming Fan, Ji Wang, Andrew E. Firth, Chune Cao, Rachel Green, Thomas E. Deverphenol:chloroform extraction and ethanol precipitation according to the manufacturer’s instructions (Promega P1280). qPCR. All qPCR experiments on RNA isolated from cultured cells followed identical cell culture and transfection

(6) Double-Stranded RNA Structural Elements Holding the Key to Translational Regulation in Cancer: The Case of Editing in RNA-Binding Motif Protein 8ACellsDecember 15, 2021Asra Abukar et al.in vitro transcription. All biotin-labeled RNA transcripts were produced in vitro using the SP6 (Promega, #P1280) or T7 (Thermo Fisher #EP0111) kit with biotin-16-UTP (Roche, 11388908910) and purified according to

(7) SARS-CoV-2 Direct Detection Without RNA Isolation With Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12Frontiers in MedicineFebruary 17, 2021Alfredo Garcia-Venzor, Bertha Rueda-Zarazua, Eduardo Marquez-Garcia, Vilma Maldonado, Angelica Moncada-Morales, Hiram Olivera, Irma Lopez, Joaquin Zuñiga, Jorge Melendez-Zajglatranscribed using RiboMAX Large Scale RNA Production System kit and following manufacturer instructions (P1280, Promega, MA, USA). The N-gene RNA copy number was determined using its molar concentration and it was

(8) Competition between two phosphatases fine-tunes Hedgehog signalingJournal of Cell BiologyFebruary 1, 2021Min Liu, Aiguo Liu, Jie Wang, Yansong Zhang, Yajuan Li, Ying Su, Alan Jian ZhuOsTIR1-SCF E3 complex. dsRNA was generated with the RiboMAX Large Scale RNA Production System (Promega; P1280) according to the manufacturer’s instructions. DNA templates targeting PpV (encoding amino acids 3–149),

(9) TGFβ-like DAF-7 acts as a systemic signal for autophagy regulation in C. elegansJournal of Cell BiologyDecember 2, 2019Yujie Zhang, Linxiang Qi, Hong Zhangsingle-strand RNA was synthesized by Large Scale RNA Production System T7 and SP6 kits (Promega, P1300 and P1280). Single-strand RNAs were then mixed and injected into animals. The F1 progeny were analyzed. Generation

(10) Hantavirus RdRp Requires a Host Cell Factor for Cap SnatchingJournal of VirologyMarch 1, 2019Subbiah Jeeva, Sheema Mir, Adrain Velasquez, Brandy A. Weathers, Aljona Leka, Sharon Wu, Ariga Tahmasian Sevarany, Mohammad MirpCDNAnsGFP (Fig. 4) were used for the synthesis of nonsense GFP mRNA using a Ribomax T7 transcription kit (P1280; Promega), as previously reported (4, 18). The synthetic mRNA was puriﬁed using an RNeasy kit (74104;

(11) Recruitment of two dyneins to an mRNA-dependent Bicaudal D transport complexeLifeJune 26, 2018Thomas E Sladewski, Neil Billington, M Yusuf Ali, Carol S Bookwalter, Hailong Lu, Elena B Krementsova, Trina A Schroer, Kathleen M Trybusdrug",paclitaxel,"Cytoskeleton, Inc",TXD01, Commercial kit,RiboMAX Large Scale RNA Production Systems,Promega,P1280, Recombinant DNA,pDyn1 (SNAPf-His-ZZ-LTLT- DYNC1H1 in pACEBac1) (Homo sapiens),Schlager et al. (2014),NCBI:NP_001367.2,Expression

(12) Chicken Interferon-induced Protein with Tetratricopeptide Repeats 5 Antagonizes Replication of RNA VirusesScientific reportsMay 1, 2018Santhakumar D, Rohaim MAMS, Hussein HA, Hawes P, Ferreira HL, Behboudi S, Iqbal M, Nair V, Arns CW, Munir M.in the presence of bioin-16-UTP using RiboMAX™ Large Scale RNA Production System-SP6 (Promega, Cat# P1280) as recommended by the manufacturer and reported previously17 with a few modifications. Briefly, a reaction

(13) Neurochemistry of neurons in the ventrolateral medulla activated by hypotension: Are the same neurons activated by glucoprivation?Journal of Comparative NeurologyJune 15, 2017Lindsay M. Parker, Sheng Le, Travis A. Wearne, Kate Hardwick, Natasha N. Kumar, Katherine J. Robinson, Simon McMullan, Ann K. GoodchildASF 3257; Epicentre Biotechnologies, Madison, WI) or SP6 (RiboMAX large scale RNA production system; P1280; Promega, Madison, WI) kits. The PPCART mRNA riboprobe was directed at base pairs 138-548 of the CART

(14) Local calcium-dependent mechanisms determine whether a cut axonal end assembles a retarded endbulb or competent growth coneExperimental NeurologyMay 5, 2009Dotan Kamber, Hadas Erez, Micha E. Spira

(15) Effective expression of the green fluorescent fusion proteins in cultured Aplysia neuronsJournal of Neuroscience MethodsMarch 14, 2003I Sahly, H Erez, A Khoutorsky, E Shapira, Micha E Spira

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RiboMAX Large Scale RNA Production Systems (SP6 and T7) From Promega
I used this kit to produce RNA for injecting into Xenopus embryos. In order to transcribe RNA, your ... Read More